Decreased TNF-α gene expression in periodontal ligature in MSG-obese rats: a possible protective effect of hypothalamic obesity against periodontal disease?

Arch Oral Biol. 2012 Mar;57(3):300-6. doi: 10.1016/j.archoralbio.2011.08.024. Epub 2011 Nov 29.

Abstract

The prevalence of obesity is increasing globally. There is evidence that the uncontrolled energetic metabolism in obese patients can accelerate periodontal disease. Therefore, the aim of this study was evaluate the possible relationship between hypothalamic obesity induced by neonatal treatment with MSG and experimental periodontal disease. Newborn male Wistar rats received subcutaneous injections in the cervical region, of 4g/Kg/day of body weight (BW) of MSG (MSG group) or hypertonic saline solution, 1.25/kg/day BW (control group, CTL). At 70 days of life periodontal disease was induced in these animals. After they were sacrificed, radiographic analyses of alveolar bone resorption and Tumor Necrosis Factor α (TNFα) gene expression in gingival tissue were performed. The neonatal treatment with MSG did not affect the concentration of plasma glucose and cholesterol (CHOL). However, plasma insulin, non-esterified fatty acids (NEFA) and triglycerides (TG) leves were higher in MSG compared with CTL group. The alveolar bone resorption was 44% lower in MSG-obese rats compared with CTL rats. In the presence of periodontal ligature, there was an increase in this parameter in all groups. The TNFα gene expression, an inflammatory marker, in periodontal tissue was similar in obese and CTL rats. The presence of ligature increased TNFα gene expression in both groups, but in a lower extension in MSG-obese rats. In conclusion these results suggested that hypothalamic obesity may produce a protective effect against periodontal disease, however further research is needed to understand the mechanisms involved in this process.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alveolar Bone Loss* / diagnostic imaging
  • Alveolar Bone Loss* / pathology
  • Alveolar Bone Loss* / prevention & control
  • Animals
  • Cholesterol / blood
  • Down-Regulation
  • Fatty Acids, Nonesterified / blood
  • Gene Expression
  • Gingiva / metabolism*
  • Hypothalamus / metabolism
  • Male
  • Obesity / blood
  • Obesity / chemically induced
  • Obesity / metabolism*
  • Radiography
  • Rats
  • Rats, Wistar
  • Real-Time Polymerase Chain Reaction
  • Sodium Glutamate
  • Triglycerides / blood
  • Tumor Necrosis Factor-alpha / genetics
  • Tumor Necrosis Factor-alpha / metabolism*

Substances

  • Fatty Acids, Nonesterified
  • Triglycerides
  • Tumor Necrosis Factor-alpha
  • Cholesterol
  • Sodium Glutamate