The effect of collagen-binding vascular endothelial growth factor on the remodeling of scarred rat uterus following full-thickness injury

Biomaterials. 2012 Feb;33(6):1801-7. doi: 10.1016/j.biomaterials.2011.11.038. Epub 2011 Dec 1.

Abstract

Serious injuries of uterine which lead to scar formation will finally result in infertility or pregnancy complications. There are few effective methods to treat such damages because of the shortage of native tissues. Vascular endothelial growth factor (VEGF) is important for the formation of new vessels and re-epithelialization of endometrium. Here we produced a collagen-binding VEGF by fusing a collagen-binding domain to the N-terminal of native VEGF. After injection into a rat scarred uterus model (partial of rat uterine horn was excised and left for scar formation) the collagen targeting VEGF promoted remodeling of the scarred uterus including the regeneration of endometrium, muscular cells, and vascularization and improved pregnancy outcomes. Thus, collagen-binding VEGF may be a pragmatic solution for the treatment of severe uterine damages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Cicatrix / pathology
  • Collagen / chemistry*
  • Endometrium / pathology
  • Female
  • Humans
  • Immunohistochemistry / methods
  • Neovascularization, Pathologic
  • Protein Structure, Tertiary
  • Rats
  • Rats, Sprague-Dawley
  • Regeneration
  • Tissue Engineering / methods*
  • Uterus / cytology
  • Uterus / metabolism*
  • Vascular Endothelial Growth Factor A / metabolism*
  • Wound Healing

Substances

  • Vascular Endothelial Growth Factor A
  • Collagen