Objective: To analyze infiltration patterns of IDH1 mutant diffuse gliomas into the brain by identification of single tumor cells applying an antibody specific to mutant IDH1 R132H protein.
Design: Immunohistochemical analysis.
Setting: University hospital.
Patients: Whole-brain and hemisphere sections of 4 patients diagnosed with diffuse glioma.
Results: Tumor cells were identified in areas that appeared inconspicuous macroscopically and at histological analysis with respect to cellularity, cellular pleomorphism, or mitotic activity in all cases.
Conclusion: Detection of single tumor cells throughout the brain demonstrates diffuse glioma to represent systemic brain disease.