Inhibition of interferon-γ-induced nitric oxide production by 10-hydroxy-trans-2-decenoic acid through inhibition of interferon regulatory factor-8 induction

Cell Immunol. 2012;273(1):73-8. doi: 10.1016/j.cellimm.2011.11.004. Epub 2011 Dec 1.

Abstract

10-Hydroxy-trans-2-decenoic acid (10H2DA) is a major lipid component of royal jelly, a honey bee secretion used to nourish the queen bee and young larvae. In this study, we examined the effect of 10H2DA on interferon (IFN)-γ-induced nitric oxide (NO) production. IFN-γ-induced NO production and activation of the inducible NO synthase promoter were significantly inhibited by 10H2DA. IFN-γ-induced phosphorylation of signal transducer and activator of transcription-1 was not affected by 10H2DA. In contrast, IFN-γ-induced tumor necrosis factor (TNF)-α production and nuclear factor (NF)-κB activation were inhibited by 10H2DA. IFN-γ-mediated induction of interferon regulatory factor (IRF)-8, but not IRF-1, was also inhibited by 10H2DA. IFN-γ-induced TNF-α production followed by activation of NF-κB is known to be essential for NO production. Together, 10H2DA inhibited IFN-γ-induced NO production by inhibiting IRF-8 induction and TNF-α production. 10H2DA might modulate IFN-γ-mediated cellular responses by inhibiting the induction of IRF-8 and IRF-8-dependent genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Enzyme Activation / immunology
  • Fatty Acids / pharmacology
  • Fatty Acids, Monounsaturated / pharmacology*
  • Interferon Regulatory Factors / antagonists & inhibitors*
  • Interferon Regulatory Factors / genetics
  • Interferon Regulatory Factors / immunology
  • Interferon-gamma / antagonists & inhibitors*
  • Interferon-gamma / immunology
  • Macrophages / drug effects*
  • Macrophages / enzymology
  • Macrophages / immunology
  • Mice
  • NF-kappa B / immunology
  • Nitric Oxide / antagonists & inhibitors
  • Nitric Oxide / biosynthesis*
  • Nitric Oxide Synthase Type II / antagonists & inhibitors
  • Nitric Oxide Synthase Type II / genetics
  • Nitric Oxide Synthase Type II / immunology
  • RNA, Messenger / chemistry
  • RNA, Messenger / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • STAT1 Transcription Factor / immunology
  • Transcription, Genetic / immunology
  • Tumor Necrosis Factor-alpha / genetics
  • Tumor Necrosis Factor-alpha / immunology

Substances

  • Fatty Acids
  • Fatty Acids, Monounsaturated
  • Interferon Regulatory Factors
  • NF-kappa B
  • RNA, Messenger
  • STAT1 Transcription Factor
  • Stat1 protein, mouse
  • Tumor Necrosis Factor-alpha
  • interferon regulatory factor-8
  • Nitric Oxide
  • 2-decenoic acid
  • Interferon-gamma
  • Nitric Oxide Synthase Type II
  • royal jelly