Determination of ABO genotypes by real-time PCR using allele-specific primers

Tomonori Muro; Junko Fujihara; Shinji Imamura; Hiroaki Nakamura; Kaori Kimura-Kataoka; Tomoko Toga; Reiko Iida; Toshihiro Yasuda; Haruo Takeshita

doi:10.1016/j.legalmed.2011.10.002

Determination of ABO genotypes by real-time PCR using allele-specific primers

Leg Med (Tokyo). 2012 Jan;14(1):47-50. doi: 10.1016/j.legalmed.2011.10.002. Epub 2011 Dec 16.

Authors

Tomonori Muro¹, Junko Fujihara, Shinji Imamura, Hiroaki Nakamura, Kaori Kimura-Kataoka, Tomoko Toga, Reiko Iida, Toshihiro Yasuda, Haruo Takeshita

Affiliation

¹ Department of Legal Medicine, Faculty of Medicine, Shimane University, Izumo 693-8501, Japan.

PMID: 22177907
DOI: 10.1016/j.legalmed.2011.10.002

Abstract

ABO grouping of biological specimens is informative for identifying victims and narrowing down suspects. In Japan and elsewhere, ABO grouping as well as DNA profiling plays an essential role in crime investigations. In the present study, we developed a new method for ABO genotyping using allele-specific primers and real-time PCR. The method allows for the detection of three single nucleotide polymorphisms (SNPs) at nucleotide positions 261, 796, and 803 in the ABO gene and the determination of six major ABO genotypes. This method required less than 2 h for accurate ABO genotyping using 2.0 ng of DNA. This method could be applicable for rapid and simple screening of forensic samples.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

ABO Blood-Group System / genetics*
Alleles
DNA Primers
Forensic Genetics / methods
Genotype*
Humans
Polymorphism, Single Nucleotide
Real-Time Polymerase Chain Reaction

Substances

ABO Blood-Group System
DNA Primers