Abstract
A mutant library consisting of hundreds of designed point and deletion mutants in the genes encoding Saccharomyces cerevisiae histones H3 and H4 is described. Incorporation of this library into a suitably engineered yeast strain (e.g., bearing a reporter of interest), and the validation of individual library members is described in detail.
MeSH terms
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Gene Library*
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Genes, Reporter / genetics
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Genetic Testing / methods*
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Histones / genetics*
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Mutation / genetics*
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Polymerase Chain Reaction
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Reproducibility of Results
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins / genetics*
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Transformation, Genetic
Substances
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Histones
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Saccharomyces cerevisiae Proteins