Abstract
Using a mouse model we show that self-complementary (sc) adeno-associated virus (AAV) vectors pseudotyped with capsids of serotypes 2, 7 or 8 induce more potent transgene product-specific CD8(+) T cell and antibody responses compared to corresponding single-stranded (ss)AAV vectors. These data suggest that the higher and more rapidly appearing amounts of transgene product achieved with scAAV vectors may increase detrimental immune responses in gene transfer recipients.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Antibodies, Viral / immunology
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CD8-Positive T-Lymphocytes / immunology
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CD8-Positive T-Lymphocytes / metabolism
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Cytokines / biosynthesis
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Dependovirus / genetics*
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Dependovirus / immunology
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Female
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Gene Transfer Techniques
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Genetic Vectors / genetics*
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Genetic Vectors / immunology
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Genome, Viral*
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Immunophenotyping
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Kinetics
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Mice
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Mice, Inbred BALB C
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Transgenes / immunology*
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gag Gene Products, Human Immunodeficiency Virus / genetics
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gag Gene Products, Human Immunodeficiency Virus / immunology
Substances
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Antibodies, Viral
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Cytokines
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gag Gene Products, Human Immunodeficiency Virus