Roles of collagen and periostin expression by cranial neural crest cells during soft palate development

J Histochem Cytochem. 2012 Jan;60(1):57-68. doi: 10.1369/0022155411427059.

Abstract

The tissue in the palatal region can be divided into the hard and the soft palates, each having a specialized function such as occlusion, speech, or swallowing. Therefore, an understanding of the mechanism of palatogenesis in relation to the function of each region is important. However, in comparison with the hard palate, there is still a lack of information about the mechanisms of soft palate development. In this study, the authors investigated the contribution of cranial neural crest (CNC) cells to development of both hard and soft palates. They also demonstrated a unique pattern of periostin expression during soft palate development, which was closely related to that of collagen type I (Col I) in palatine aponeurosis. Furthermore, organ culture analysis showed that exogenous transforming growth factor-β (TGF-β) induced the expression of both periostin and Col I. These novel patterns of expression in the extracellular matrix (ECM) induced by CNC cells suggest that these cells may help to determine the character of both the hard and soft palates through ECM induction. TGF-β signaling appears to be one of the mediators of Col I and periostin expression in the formation of functional structures during soft palate development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Cell Adhesion Molecules / metabolism*
  • Collagen Type I / metabolism*
  • Extracellular Matrix / metabolism
  • Fluorescent Antibody Technique
  • Mesoderm / cytology
  • Mesoderm / metabolism
  • Mice
  • Mice, Transgenic
  • Neural Crest / cytology
  • Neural Crest / metabolism*
  • Organ Culture Techniques
  • Palate / embryology
  • Palate / growth & development
  • Palate / metabolism*
  • Palate, Hard / embryology
  • Palate, Hard / growth & development
  • Palate, Hard / metabolism
  • Signal Transduction
  • Transforming Growth Factor beta / pharmacology
  • Transforming Growth Factor beta / physiology

Substances

  • Cell Adhesion Molecules
  • Collagen Type I
  • Postn protein, mouse
  • Transforming Growth Factor beta