Molecular diagnosis of subcutaneous Pythium insidiosum infection by use of PCR screening and DNA sequencing

J Clin Microbiol. 2012 Apr;50(4):1480-3. doi: 10.1128/JCM.06126-11. Epub 2012 Jan 11.

Abstract

Pythium insidiosum is an emerging human pathogen classified among brown algae and diatoms that can cause significant morbidity and mortality in otherwise healthy individuals. Here we describe a pediatric patient with pythiosis acquired in the southern United States, diagnosed by molecular screening and DNA sequencing of internal transcribed spacer region 1.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Amputation, Surgical
  • Base Sequence
  • Female
  • Humans
  • Leg / parasitology*
  • Leg / surgery
  • Molecular Diagnostic Techniques
  • Molecular Sequence Data
  • Molecular Typing
  • Polymerase Chain Reaction*
  • Pythiosis / diagnosis*
  • Pythiosis / parasitology
  • Pythium*
  • Sequence Analysis, DNA

Associated data

  • GENBANK/JQ305799
  • GENBANK/JQ305800
  • GENBANK/JQ305801