HDL-associated enzymes and proteins in hemodialysis patients

Clin Biochem. 2012 Feb;45(3):243-8. doi: 10.1016/j.clinbiochem.2011.12.008. Epub 2011 Dec 20.

Abstract

Objectives: To evaluate HDL-associated proteins and enzymes and their relation with lipoprotein profile and inflammatory markers in chronic renal patients on hemodialysis.

Design and methods: We studied 53 patients under hemodialysis and 32 healthy subjects as controls. We compared plasma lipids, Apoprotein-AI and hs-CRP, as a marker of chronic inflammation. We evaluated proteins and enzymes associated to HDL, involved in several points of lipoprotein metabolism: CETP, paraoxonase and LpPLA2 activities. Hepatic lipase was measured in postheparin plasma.

Results: Patients showed higher triglycerides and lower LDL-, HDL- and total-cholesterol than controls (p<0.05). Also, in comparison with controls, Apoprotein-AI, paraoxonase and hepatic lipase were lower, while CETP was higher (p<0.03). LpPLA2 did not show changes between groups.

Conclusion: Beyond plasma lipid-lipoprotein profile, other factors could contribute to induce a pro-oxidative and pro-inflammatory status. The protective role of HDL does not only depend on its concentration, but also on its functionality.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • C-Reactive Protein / metabolism
  • Case-Control Studies
  • Cholesterol Ester Transfer Proteins / blood
  • Enzymes / blood*
  • Female
  • Humans
  • Lipoproteins, HDL / blood*
  • Male
  • Middle Aged
  • Renal Dialysis*
  • Statistics, Nonparametric

Substances

  • Cholesterol Ester Transfer Proteins
  • Enzymes
  • Lipoproteins, HDL
  • C-Reactive Protein