Abstract
Anti-tumor activity and mechanism of matrine is evaluated and investigated. MTT assay showed that the matrine was able to inhibit gastric cancer cell line MNK45 in a dose-dependent manner. The concentration required for 50% inhibition (IC50) was found to be 540 μg/ml. This anti-tumor function was achieved through modulation of the NF-κB, XIAP, CIAP, and p-ERK proteins expression in cell line MNK45. By western blot analysis, we found that expression of NF-κB, XIAP, CIAP, and p-ERK proteins in cell line MNK45 would vary with varying concentration of matrine. These protein interactions possibly play a pivotal role in the regulation of apoptosis, for which further detailed analyzes are need. These results overall indicate that matrine can be used as an effective anti-tumor agent in therapy of gastric cancer.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaloids / pharmacology*
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Alkaloids / therapeutic use
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Antineoplastic Agents, Phytogenic / pharmacology*
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Antineoplastic Agents, Phytogenic / therapeutic use
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Drug Screening Assays, Antitumor
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Extracellular Signal-Regulated MAP Kinases / metabolism
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Humans
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I-kappa B Proteins / metabolism
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Matrines
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NF-kappa B / metabolism
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Phosphorylation / drug effects
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Protein Subunits / metabolism
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Quinolizines / pharmacology*
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Quinolizines / therapeutic use
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Stomach Neoplasms / drug therapy
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Stomach Neoplasms / pathology*
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X-Linked Inhibitor of Apoptosis Protein / metabolism
Substances
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Alkaloids
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Antineoplastic Agents, Phytogenic
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I-kappa B Proteins
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NF-kappa B
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Protein Subunits
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Quinolizines
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X-Linked Inhibitor of Apoptosis Protein
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Extracellular Signal-Regulated MAP Kinases
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Matrines