Isolated leptomeningeal infiltration of a primary CNS B-cell lymphoma diagnosed by flow cytometry and confirmed by necropsy

Acta Neurol Scand. 2012 Sep;126(3):e11-6. doi: 10.1111/j.1600-0404.2011.01630.x. Epub 2011 Dec 26.

Abstract

Background: The diagnosis of the isolated leptomeningeal involvement of a primary central nervous system B-cell lymphoma without parenchyma lesions may be difficult. Patients with leptomeningeal meningeosis lymphomatosa can present with various neurologic deficits.

Aims of the study: To demonstrate the impact of cerebrospinal fluid (CSF) flow cytometry in the diagnosis of an isolated leptomeningeal manifestation of B-cell lymphoma by presenting an interesting case report.

Methods: Flow cytometric analysis of B-cell monoclonality of the CSF was performed as complementary diagnostic procedure in addition to CSF cytology. Final diagnosis was confirmed by necropsy.

Results: We suspected isolated leptomeningeal manifestation of B-cell lymphoma with palsy of the VI and VII cranial nerves in a 79-year-old male, because of mononuclear pleocytosis in CSF. Interestingly, the decisive diagnostic hint was given by implementation of flow cytometry of the CSF. Diagnosis was confirmed by postmortem autopsy.

Conclusion: Our case shows that flow cytometry of the CSF in addition to conventional CSF cytology has the potential to accelerate diagnosis of lymphomeningeal infiltration of B-cell lymphoma.

Publication types

  • Case Reports

MeSH terms

  • Aged
  • Central Nervous System Neoplasms / cerebrospinal fluid
  • Central Nervous System Neoplasms / pathology*
  • Cytological Techniques
  • Flow Cytometry*
  • Humans
  • Lymphoma, Non-Hodgkin / cerebrospinal fluid
  • Lymphoma, Non-Hodgkin / pathology*
  • Male
  • Meningeal Neoplasms / cerebrospinal fluid
  • Meningeal Neoplasms / diagnosis*
  • Meningeal Neoplasms / secondary*
  • Necrosis / diagnosis