Photo catalogue for the classification of cell colonies in the Syrian hamster embryo (SHE) cell transformation assay at pH 6.7

Susanne Bohnenberger; Shannon Wilson Bruce; Thorsten Kunkelmann; Kamala Pant; Sabine Perschbacher; Karl-Rainer Schwind; Jamie Sly; Albrecht Poth

doi:10.1016/j.mrgentox.2011.12.013

Photo catalogue for the classification of cell colonies in the Syrian hamster embryo (SHE) cell transformation assay at pH 6.7

Mutat Res. 2012 Apr 11;744(1):82-96. doi: 10.1016/j.mrgentox.2011.12.013. Epub 2011 Dec 23.

Authors

Susanne Bohnenberger¹, Shannon Wilson Bruce, Thorsten Kunkelmann, Kamala Pant, Sabine Perschbacher, Karl-Rainer Schwind, Jamie Sly, Albrecht Poth

Affiliation

¹ Harlan Cytotest Cell Research, Rossdorf, Germany. [email protected]

PMID: 22212200
DOI: 10.1016/j.mrgentox.2011.12.013

Abstract

This catalogue is a display of Syrian hamster embryo (SHE) cell colony photos representative of the cell transformation assay (CTA) carried out at pH 6.7. It is intended as a visual aid for the identification and the scoring of cell colonies in the conduct of the assay. A proper training from experienced personnel together with the protocol reported in this issue and the present photo catalogue will support method transfer and consistency in the assay results.

MeSH terms

Animals
Audiovisual Aids*
Carcinogenicity Tests / methods*
Carcinogens / toxicity
Catalogs as Topic*
Cell Transformation, Neoplastic*
Cells, Cultured
Cricetinae
Hydrogen-Ion Concentration
Mesocricetus*
Photography*

Substances

Carcinogens