Simultaneous purification and site-specific modification of pyrroline-carboxy-lysine proteins

Hsien-Po Chiu; Jan Grünewald; Xueshi Hao; Ansgar Brock; Linda Okach; Tetsuo Uno; Bernhard H Geierstanger

doi:10.1002/cbic.201100684

Simultaneous purification and site-specific modification of pyrroline-carboxy-lysine proteins

Chembiochem. 2012 Feb 13;13(3):364-6. doi: 10.1002/cbic.201100684. Epub 2012 Jan 5.

Authors

Hsien-Po Chiu¹, Jan Grünewald, Xueshi Hao, Ansgar Brock, Linda Okach, Tetsuo Uno, Bernhard H Geierstanger

Affiliation

¹ Genomics Institute of the Novartis Research Foundation, 10675 John-Jay-Hopkins Drive, San Diego, CA 92121, USA.

PMID: 22223621
DOI: 10.1002/cbic.201100684

Abstract

Sticky residue: Pyrroline-carboxy-lysine (Pcl) can be readily incorporated into proteins expressed in E. coli and mammalian cells by using the pyrrolysyl tRNA/tRNA synthetase pair. Pcl can be used as a single amino acid purification tag and can be site-specifically modified with functional probes during the elution process.

MeSH terms

Benzaldehydes / chemistry
Binding Sites
Lysine / analogs & derivatives*
Lysine / chemistry
Lysine / metabolism
Molecular Structure
Proteins / chemistry*
Proteins / isolation & purification*

Substances

2-amino-6-(3,4-dihydro-2H-pyrrole-2-carboxamido)hexanoic acid
Benzaldehydes
Proteins
2-aminobenzaldehyde
Lysine