Inflammatory changes in adipose tissue enhance expression of GPR84, a medium-chain fatty acid receptor: TNFα enhances GPR84 expression in adipocytes

FEBS Lett. 2012 Feb 17;586(4):368-72. doi: 10.1016/j.febslet.2012.01.001. Epub 2012 Jan 10.

Abstract

In this study we aimed to identify the physiological roles of G protein-coupled receptor 84 (GPR84) in adipose tissue, together with medium-chain fatty acids (MCFAs), the specific ligands for GPR84. In mice, high-fat diet up-regulated GPR84 expression in fat pads. In 3T3-L1 adipocytes, co-culture with a macrophage cell line, RAW264, or TNFα remarkably enhanced GPR84 expression. In the presence of TNFα, MCFAs down-regulated adiponectin mRNA expression in 3T3-L1 adipocytes. Taken together, our results suggest that GPR84 emerges in adipocytes in response to TNFα from infiltrating macrophages and exacerbates the vicious cycle between adiposity and diabesity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / drug effects
  • Adipocytes / metabolism
  • Adiponectin / genetics
  • Adipose Tissue / metabolism*
  • Animals
  • Cell Line
  • Coculture Techniques
  • Decanoic Acids / pharmacology
  • Fatty Acids / metabolism
  • Gene Expression / drug effects
  • Inflammation / genetics*
  • Inflammation / metabolism
  • Ligands
  • Macrophages / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, G-Protein-Coupled / genetics*
  • Receptors, G-Protein-Coupled / metabolism
  • Tumor Necrosis Factor-alpha / pharmacology*

Substances

  • Adiponectin
  • Adipoq protein, mouse
  • Decanoic Acids
  • Fatty Acids
  • Gpr84 protein, mouse
  • Ligands
  • RNA, Messenger
  • Receptors, G-Protein-Coupled
  • Tumor Necrosis Factor-alpha
  • decanoic acid