Kistimonas scapharcae sp. nov., isolated from a dead ark clam (Scapharca broughtonii), and emended description of the genus Kistimonas

Int J Syst Evol Microbiol. 2012 Dec;62(Pt 12):2865-2869. doi: 10.1099/ijs.0.038422-0. Epub 2012 Jan 13.

Abstract

A Gram-negative, motile, facultatively anaerobic rod, designated A36(T), was isolated from a dead ark clam found on the south coast of Korea. The isolate was catalase- and oxidase-negative. 16S rRNA gene sequence analysis indicated that strain A36(T) was most closely related to Kistimonas asteriae KMD 001(T), with which it shared 98.2% 16S rRNA gene sequence similarity. Strain A36(T) grew optimally at 30-37 °C, with 1% (w/v) NaCl and at pH 8.0. The major respiratory quinone was ubiquinone-9 (Q-9). The major polar lipids were phosphatidylserine, phosphoethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids were summed feature 3 (comprising C(16:1)ω7c and/or iso-C(15) 2-OH) and C(16:0). The genomic DNA G+C content was 47.3 mol%. DNA-DNA relatedness between the isolate and K. asteriae JCM 15607(T) was <25 ± 3%. Strain A36(T) represents a novel species of the genus Kistimonas, for which the name Kistimonas scapharcae sp. nov. is proposed. The type strain is A36(T) ( = KACC 16204(T) = JCM 17805(T)). An emended description of the genus Kistimonas is also provided.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / analysis
  • Gammaproteobacteria / classification*
  • Gammaproteobacteria / genetics
  • Gammaproteobacteria / isolation & purification
  • Molecular Sequence Data
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Republic of Korea
  • Scapharca / microbiology*
  • Sequence Analysis, DNA
  • Ubiquinone / analysis

Substances

  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S
  • Ubiquinone
  • ubiquinone 9

Associated data

  • GENBANK/JF811908