Cell surface biotinylation by azaelectrocyclization: easy-handling and versatile approach for living cell labeling

Katsunori Tanaka; Satomi Yokoi; Koji Morimoto; Takayuki Iwata; Yuka Nakamoto; Kaori Nakayama; Koichi Koyama; Takeshi Fujiwara; Koichi Fukase

doi:10.1016/j.bmc.2011.12.043

Cell surface biotinylation by azaelectrocyclization: easy-handling and versatile approach for living cell labeling

Bioorg Med Chem. 2012 Mar 15;20(6):1865-8. doi: 10.1016/j.bmc.2011.12.043. Epub 2011 Dec 30.

Authors

Katsunori Tanaka¹, Satomi Yokoi, Koji Morimoto, Takayuki Iwata, Yuka Nakamoto, Kaori Nakayama, Koichi Koyama, Takeshi Fujiwara, Koichi Fukase

Affiliation

¹ Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka-shi, Osaka 560-0043, Japan.

PMID: 22257530
DOI: 10.1016/j.bmc.2011.12.043

Abstract

Versatile method for living cell labeling has been established. Cell surfaces are initially biotinylated by azaelectrocyclization, and then treated with the fluorescence-labeled avidin or the anti-biotin antibody.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies / immunology
Avidin / chemistry*
Biotin / chemistry*
Biotin / immunology
Biotinylation
Cell Line
Cell Membrane / chemistry*
Cell Membrane / ultrastructure
Cell Survival
Fluorescent Dyes / chemistry*
HeLa Cells
Humans
Microscopy, Confocal / methods
Staining and Labeling / methods*

Substances

Antibodies
Fluorescent Dyes
Avidin
Biotin