Evaluation of adding a second marker to overcome Staphylococcus aureus spa typing homoplasies

P Basset; U Nübel; W Witte; D S Blanc

doi:10.1128/JCM.00664-11

Evaluation of adding a second marker to overcome Staphylococcus aureus spa typing homoplasies

J Clin Microbiol. 2012 Apr;50(4):1475-7. doi: 10.1128/JCM.00664-11. Epub 2012 Jan 18.

Authors

P Basset¹, U Nübel, W Witte, D S Blanc

Affiliation

¹ Hospital Preventive Medicine Service, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland. [email protected]

Abstract

The utility of sequencing a second highly variable locus in addition to the spa gene (e.g., double-locus sequence typing [DLST]) was investigated to overcome limitations of a Staphylococcus aureus single-locus typing method. Although adding a second locus seemed to increase discriminatory power, it was not sufficient to definitively infer evolutionary relationships within a single multilocus sequence type (ST-5).

Publication types

Evaluation Study

MeSH terms

Adhesins, Bacterial / genetics
Antigens, Bacterial / genetics
Bayes Theorem
Evolution, Molecular
Genetic Markers*
Haplotypes
Markov Chains
Models, Genetic
Monte Carlo Method
Multilocus Sequence Typing*
Phylogeny
Polymorphism, Single Nucleotide
Staphylococcus aureus / classification
Staphylococcus aureus / genetics*

Substances

Adhesins, Bacterial
Antigens, Bacterial
Genetic Markers
clfB protein, Staphylococcus aureus
streptococcal protective antigen