Analgesic, anti-inflammatory, and CNS depressant activities of new constituents of Nepeta clarkei

Fitoterapia. 2012 Apr;83(3):593-8. doi: 10.1016/j.fitote.2012.01.002. Epub 2012 Jan 14.

Abstract

Two new pentacyclic triterpenes named kirmanoic acid (1) and kurramanoic acid (2) have been isolated from the chloroform-soluble portion of the whole plant of Nepeta clarkei Hook. The structures of the two new compounds were assigned on the basis of their ¹H and ¹³C NMR spectra including two-dimensional NMR techniques such as COSY, HMQC, and HMBC experiments. Kirmanoic acid (1) was investigated for analgesic, anti-inflammatory, and CNS depressant activities. Interestingly kirmanoic acid (1) showed strong analgesic activity than standard drug in acetic induced writhing and formalin tests. Similarly kirmanoic acid (1) also showed strong anti-inflammatory activity than its standard drug. The gross behavioral study of kirmanoic acid (1) revealed that it exhibited mild CNS stimulant and muscle relaxant in the mice. Compound 1 showed a slight increase in Locomotor activity and possesses the antidepressant effect.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetic Acid
  • Analgesics / isolation & purification
  • Analgesics / pharmacology
  • Analgesics / therapeutic use*
  • Animals
  • Anti-Inflammatory Agents / isolation & purification
  • Anti-Inflammatory Agents / pharmacology
  • Anti-Inflammatory Agents / therapeutic use*
  • Antidepressive Agents / isolation & purification
  • Antidepressive Agents / pharmacology
  • Antidepressive Agents / therapeutic use
  • Behavior, Animal / drug effects*
  • Central Nervous System Depressants / isolation & purification
  • Central Nervous System Depressants / pharmacology
  • Central Nervous System Depressants / therapeutic use*
  • Central Nervous System Stimulants / isolation & purification
  • Central Nervous System Stimulants / pharmacology
  • Central Nervous System Stimulants / therapeutic use
  • Formaldehyde
  • Male
  • Mice
  • Mice, Inbred Strains
  • Molecular Structure
  • Nepeta / chemistry*
  • Neuromuscular Agents / isolation & purification
  • Neuromuscular Agents / pharmacology
  • Neuromuscular Agents / therapeutic use
  • Pain / chemically induced
  • Pain / drug therapy
  • Phytotherapy*
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use
  • Rats
  • Rats, Wistar
  • Triterpenes / chemistry
  • Triterpenes / isolation & purification
  • Triterpenes / pharmacology
  • Triterpenes / therapeutic use*

Substances

  • Analgesics
  • Anti-Inflammatory Agents
  • Antidepressive Agents
  • Central Nervous System Depressants
  • Central Nervous System Stimulants
  • Neuromuscular Agents
  • Plant Extracts
  • Triterpenes
  • kirmanoic acid
  • Formaldehyde
  • Acetic Acid