Magnetic pH-responsive nanogels as multifunctional delivery tools for small interfering RNA (siRNA) molecules and iron oxide nanoparticles (IONPs)

Alberto Curcio; Roberto Marotta; Andreas Riedinger; Domenico Palumberi; Andrea Falqui; Teresa Pellegrino

doi:10.1039/c2cc17223b

Magnetic pH-responsive nanogels as multifunctional delivery tools for small interfering RNA (siRNA) molecules and iron oxide nanoparticles (IONPs)

Chem Commun (Camb). 2012 Feb 28;48(18):2400-2. doi: 10.1039/c2cc17223b. Epub 2012 Jan 23.

Authors

Alberto Curcio¹, Roberto Marotta, Andreas Riedinger, Domenico Palumberi, Andrea Falqui, Teresa Pellegrino

Affiliation

¹ Istituto Italiano di Tecnologia, via Morego 30, 161613, Genoa, Italy.

PMID: 22266784
DOI: 10.1039/c2cc17223b

Abstract

We here exploit pH-responsive nanogels as carriers to deliver functional anti-GFP siRNA and superparamagnetic IONPs to HeLa-GFP cells. The siRNA release via pH-mediated endosomal escape is shown. The IONPs act first as magnetofection agents to boost cellular uptake and then as probes to track the release mechanism by electron microscopy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biological Transport
Drug Carriers / chemistry*
Ferric Compounds / chemistry*
Ferric Compounds / metabolism*
Gels
HeLa Cells
Humans
Hydrogen-Ion Concentration
Intracellular Space / metabolism
Magnetic Phenomena*
Nanoparticles / chemistry*
RNA, Small Interfering / metabolism*

Substances

Drug Carriers
Ferric Compounds
Gels
RNA, Small Interfering
ferric oxide