Identification of hydrophobic tags for the degradation of stabilized proteins

Hyun Seop Tae; Thomas B Sundberg; Taavi K Neklesa; Devin J Noblin; Jeffrey L Gustafson; Anke G Roth; Kanak Raina; Craig M Crews

doi:10.1002/cbic.201100793

Identification of hydrophobic tags for the degradation of stabilized proteins

Chembiochem. 2012 Mar 5;13(4):538-41. doi: 10.1002/cbic.201100793. Epub 2012 Jan 23.

Authors

Hyun Seop Tae¹, Thomas B Sundberg, Taavi K Neklesa, Devin J Noblin, Jeffrey L Gustafson, Anke G Roth, Kanak Raina, Craig M Crews

Affiliation

¹ Department of Molecular Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT 06511, USA.

Abstract

New HyTs are a knockout: we previously reported that labeling HaloTag proteins with low molecular weight hydrophobic tags (HyTs) leads to targeted degradation of HaloTag fusion proteins. In this report, we employed a chemical approach to extend this hydrophobic tagging methodology to highly stabilized proteins by synthesizing and evaluating a library of HyTs, which led to the identification of HyT36.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

HEK293 Cells
Humans
Hydrophobic and Hydrophilic Interactions
Molecular Structure
Recombinant Fusion Proteins / chemistry
Recombinant Fusion Proteins / metabolism*

Substances

Recombinant Fusion Proteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding