Genomic characterization of a cell-culture-adapted Korean human G9P[8] rotavirus, CAU05-202

Arch Virol. 2012 Apr;157(4):753-9. doi: 10.1007/s00705-012-1232-z. Epub 2012 Jan 25.

Abstract

The human rotavirus G9 strain is the fifth most common rotavirus worldwide. A human rotavirus G9P[8] strain CAU05-202 was isolated from a young child with diarrhea using a cell culture system, and its major gene sequences were determined. Phylogenetic analysis of the VP7 gene revealed that CAU05-202 clustered into genetic lineage III-d and was most closely related to G9 rotaviruses from Turkey (strain GUH13) and Sri Lanka (strain 05SLC056 and 05SLC057). VP4 and NSP4 gene analysis showed that CAU05-202 belongs to the P[8]-3 lineage and genotype B, respectively. In addition, CAU05-202 has a long RNA electropherotype, supported by VP6 gene analysis, which is clearly associated with subgroup II specificity. Analysis of the G9 rotavirus strain CAU05-202 provides information concerning the genetic relationships among global rotavirus G9 strains, suggesting that closely related G9 strains are persistent and widespread in Asian countries.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Viral / genetics
  • Capsid Proteins / genetics
  • Cell Culture Techniques
  • Cluster Analysis
  • Diarrhea / virology
  • Genome, Viral*
  • Genotype
  • Glycoproteins / genetics
  • Humans
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Viral / genetics*
  • Republic of Korea
  • Rotavirus / genetics*
  • Rotavirus / growth & development*
  • Rotavirus / isolation & purification
  • Rotavirus Infections / virology
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Serial Passage
  • Toxins, Biological / genetics
  • Viral Nonstructural Proteins / genetics

Substances

  • Antigens, Viral
  • Capsid Proteins
  • Glycoproteins
  • NS28 protein, rotavirus
  • RNA, Viral
  • Toxins, Biological
  • VP4 protein, Rotavirus
  • VP6 protein, Rotavirus
  • VP7 protein, Rotavirus
  • Viral Nonstructural Proteins

Associated data

  • GENBANK/EF059922
  • GENBANK/EF059923
  • GENBANK/EF059924
  • GENBANK/EU556223