Abstract
Identifying cellular reservoirs of human immunodeficiency virus type 1 (HIV-1) in patients on antiretroviral therapy (ART) is critical to finding a cure for HIV-1. In addition to resting CD4(+) T cells, CD34(+) hematopoietic progenitor cells have been proposed as another reservoir. We obtained bone marrow aspirates from 11 patients on ART who had undetectable plasma HIV-1 RNA. HIV-1 DNA was detected in CD4(+) T cells from peripheral blood in all patients and from bone marrow cellular fractions containing T cells in most patients. We did not find HIV-1 DNA in highly purified CD34(+) populations using either a sensitive real-time polymerase chain reaction assay or a coculture assay for replication-competent HIV-1.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Anti-Retroviral Agents / therapeutic use
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Antigens, CD34 / analysis
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Bone Marrow / chemistry
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Bone Marrow / virology
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Bone Marrow Cells / chemistry
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Bone Marrow Cells / virology*
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CD4-Positive T-Lymphocytes / chemistry
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CD4-Positive T-Lymphocytes / virology*
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DNA, Viral / isolation & purification*
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HIV Infections / immunology
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HIV-1 / isolation & purification
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HIV-1 / pathogenicity
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HIV-1 / physiology
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Hematopoietic Stem Cells / chemistry
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Hematopoietic Stem Cells / virology*
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Humans
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Male
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Middle Aged
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Real-Time Polymerase Chain Reaction
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Virus Replication
Substances
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Anti-Retroviral Agents
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Antigens, CD34
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DNA, Viral