Lipidic phase membrane protein serial femtosecond crystallography

Nat Methods. 2012 Jan 29;9(3):263-5. doi: 10.1038/nmeth.1867.

Abstract

X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Crystallography, X-Ray / methods*
  • Lipid Bilayers / chemistry*
  • Membrane Proteins / chemistry*
  • Membrane Proteins / ultrastructure*
  • Protein Binding
  • Protein Conformation / radiation effects
  • X-Rays

Substances

  • Lipid Bilayers
  • Membrane Proteins