Transcription-independent role of Bach1 in mitosis through a nuclear exporter Crm1-dependent mechanism

FEBS Lett. 2012 Feb 17;586(4):448-54. doi: 10.1016/j.febslet.2012.01.028. Epub 2012 Jan 28.

Abstract

The transcriptional repressor Bach1 mediates various stress responses. Despite its role in transcription, Bach1 is predominantly exported to the cytoplasm in a Crm1-dependent manner, but the functional role of its cytoplasmic retention is still unclear. We found that Bach1 was also excluded from mitotic chromatin by a C-terminal cytoplasmic localization sequence dependent and leptomycin B sensitive process. Bach1 depletion resulted in disordered mitotic chromosome alignment, which was rescued by Bach1 mutants lacking the BTB or DNA binding domains, suggesting its transcription-independent mechanism. We thus revealed a novel role of Bach1 in the regulation of mitotic chromosome dynamics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Basic-Leucine Zipper Transcription Factors / antagonists & inhibitors
  • Basic-Leucine Zipper Transcription Factors / chemistry
  • Basic-Leucine Zipper Transcription Factors / genetics*
  • Basic-Leucine Zipper Transcription Factors / metabolism*
  • Chromosome Pairing / drug effects
  • Chromosome Pairing / genetics
  • Chromosome Pairing / physiology
  • DNA Primers / genetics
  • Exportin 1 Protein
  • Fanconi Anemia Complementation Group Proteins / antagonists & inhibitors
  • Fanconi Anemia Complementation Group Proteins / chemistry
  • Fanconi Anemia Complementation Group Proteins / genetics*
  • Fanconi Anemia Complementation Group Proteins / metabolism*
  • Fatty Acids, Unsaturated / pharmacology
  • Gene Knockdown Techniques
  • HEK293 Cells
  • Humans
  • Karyopherins / genetics*
  • Karyopherins / metabolism*
  • Mice
  • Mitosis / genetics*
  • Mitosis / physiology*
  • Models, Molecular
  • Molecular Sequence Data
  • Protein Structure, Tertiary
  • Pyrimidines / pharmacology
  • RNA, Small Interfering / genetics
  • Receptors, Cytoplasmic and Nuclear / genetics*
  • Receptors, Cytoplasmic and Nuclear / metabolism*
  • Sequence Homology, Amino Acid
  • Thiones / pharmacology
  • Transcription, Genetic

Substances

  • BACH1 protein, human
  • Bach1 protein, mouse
  • Basic-Leucine Zipper Transcription Factors
  • DNA Primers
  • Fanconi Anemia Complementation Group Proteins
  • Fatty Acids, Unsaturated
  • Karyopherins
  • Pyrimidines
  • RNA, Small Interfering
  • Receptors, Cytoplasmic and Nuclear
  • Thiones
  • monastrol
  • leptomycin B