Pyrosequencing analysis of 16S rRNA genes was used to examine impacts of elevated CO(2) (eCO(2)) on soil microbial communities from 12 replicates each from ambient CO(2) (aCO(2)) and eCO(2) settings. The results suggest that the soil microbial community composition and structure significantly altered under conditions of eCO(2), which was closely associated with soil and plant properties.