Abstract
To clarify the relationship between the myocardial accumulation of 125I-15-(p-iodophenyl)-3-(R,S)-methylpentadecanoic acid (BMIPP) and intracellular adenosine-5'-triphosphate (ATP) content, the effect of 2,4-dinitrophenol (DNP, an electron transport uncoupler) on myocardial BMIPP accumulation was studied, in comparison with that of thallium-201-chloride (201Tl-Cl). In the mouse myocardium, DNP decreased the intracellular ATP and ADP levels, without affecting either acyl-CoA synthetase activity or the level of CoA-SH. Following treatment with DNP, decreases in myocardial BMIPP accumulation correlated well with those of ATP, while 201Tl-Cl showed slightly increased accumulation in the myocardium. Thus, in some diseases, BMIPP may be useful in evaluating myocardial ATP levels.
MeSH terms
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2,4-Dinitrophenol
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Adenosine Diphosphate / analysis
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Adenosine Monophosphate / analysis
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Adenosine Triphosphate / analysis*
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Animals
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Coenzyme A / analysis
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Coenzyme A Ligases / metabolism
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Dinitrophenols / pharmacology
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Fatty Acids*
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Heart / diagnostic imaging*
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Heart / drug effects
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Iodine Radioisotopes
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Iodobenzenes / chemistry
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Iodobenzenes / pharmacokinetics*
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Male
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Mice
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Myocardium / chemistry
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Myocardium / metabolism*
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Radionuclide Imaging
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Thallium / pharmacokinetics
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Thallium Radioisotopes / pharmacokinetics
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Tissue Distribution
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Uncoupling Agents / pharmacology
Substances
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Dinitrophenols
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Fatty Acids
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Iodine Radioisotopes
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Iodobenzenes
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Thallium Radioisotopes
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Uncoupling Agents
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iodofiltic acid
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thallium chloride
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Adenosine Monophosphate
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Adenosine Diphosphate
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Adenosine Triphosphate
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Thallium
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Coenzyme A Ligases
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2,4-Dinitrophenol
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Coenzyme A