Characterization of hydrogen sulfide and its synthases, cystathionine β-synthase and cystathionine γ-lyase, in human prostatic tissue and cells

Urology. 2012 Feb;79(2):483.e1-5. doi: 10.1016/j.urology.2011.10.013.

Abstract

Objective: To investigate hydrogen sulfide and its synthases, cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE), in human prostatic tissue and cells.

Methods: CBS and CSE in human prostatic tissue and cells were located using immunostaining. Western blot and a sulfur-sensitive electrode were used to evaluate the expression levels and catalytic activity of CBS and CSE. We analyzed the association between dihydrotestosterone-added or hormone-reduced medium-induced CBS/CSE protein levels with androgen receptor levels in prostate cancer lines. All experiments were repeated ≥3 times.

Results: Endogenous hydrogen sulfide and its synthases existed in various areas of human prostatic tissue and cells. Cell activity and CBS/CSE protein levels were greatest in the androgen-dependent prostate cancer cell LNCaP among all cells and downregulated by dihydrotestosterone.

Conclusion: Hydrogen sulfide and its synthases in human prostatic tissue and cells were modulated by dihydrotestosterone, which could suggest a potential therapy for prostatic disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / chemistry
  • Adenocarcinoma / enzymology
  • Adenocarcinoma / pathology
  • Androgens
  • Cell Line / chemistry
  • Cell Line / drug effects
  • Cell Line / enzymology
  • Cell Line, Tumor / chemistry
  • Cell Line, Tumor / drug effects
  • Cell Line, Tumor / enzymology
  • Culture Media / pharmacology
  • Cystathionine beta-Synthase / analysis*
  • Cystathionine beta-Synthase / biosynthesis
  • Cystathionine beta-Synthase / genetics
  • Cystathionine gamma-Lyase / analysis*
  • Cystathionine gamma-Lyase / biosynthesis
  • Cystathionine gamma-Lyase / genetics
  • Dihydrotestosterone / pharmacology
  • Down-Regulation / drug effects
  • Enzyme Induction / drug effects
  • Epithelial Cells / chemistry
  • Epithelial Cells / drug effects
  • Epithelial Cells / enzymology
  • Gene Expression Regulation, Neoplastic / drug effects
  • Humans
  • Hydrogen Sulfide / analysis*
  • Male
  • Neoplasm Proteins / analysis
  • Neoplasm Proteins / biosynthesis
  • Neoplasm Proteins / genetics
  • Neoplasms, Hormone-Dependent / chemistry
  • Neoplasms, Hormone-Dependent / enzymology
  • Neoplasms, Hormone-Dependent / pathology
  • Prostate / chemistry*
  • Prostate / cytology
  • Prostate / enzymology
  • Prostatic Neoplasms / chemistry
  • Prostatic Neoplasms / enzymology
  • Prostatic Neoplasms / pathology
  • Receptors, Androgen / analysis
  • Receptors, Androgen / biosynthesis
  • Receptors, Androgen / genetics
  • Stromal Cells / chemistry
  • Stromal Cells / drug effects
  • Stromal Cells / enzymology

Substances

  • Androgens
  • Culture Media
  • Neoplasm Proteins
  • Receptors, Androgen
  • Dihydrotestosterone
  • Cystathionine beta-Synthase
  • Cystathionine gamma-Lyase
  • Hydrogen Sulfide