Abstract
Triptolide is a diterpenoid triepoxide derived from the traditional Chinese medical herb Tripterygium wilfordii. In the present study, we demonstrated that this phytochemical attenuated colon cancer growth in vitro and in vivo. Using a proteomic approach, we found that 14-3-3 epsilon, a cell cycle- and apoptosis-related protein, was altered in colon cancer cells treated with triptolide. In this regard, triptolide induced cleavage and perinuclear translocation of 14-3-3 epsilon. Taken together, our findings suggest that triptolide may merit investigation as a potential therapeutic agent for colon cancer, and its anticancer action may be associated with alteration of 14-3-3 epsilon.
Copyright © 2012 John Wiley & Sons, Ltd.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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14-3-3 Proteins / metabolism*
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Amino Acid Sequence
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Animals
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Antineoplastic Agents, Alkylating / isolation & purification
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Antineoplastic Agents, Alkylating / pharmacology*
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Antineoplastic Agents, Alkylating / therapeutic use
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Colonic Neoplasms / drug therapy
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Colonic Neoplasms / metabolism
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Colonic Neoplasms / pathology
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Diterpenes / isolation & purification
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Diterpenes / pharmacology*
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Diterpenes / therapeutic use
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Electrophoresis, Gel, Two-Dimensional
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Epoxy Compounds / isolation & purification
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Epoxy Compounds / pharmacology
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Epoxy Compounds / therapeutic use
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Humans
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Medicine, Chinese Traditional
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Mice
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Mice, Nude
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Molecular Sequence Data
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Phenanthrenes / isolation & purification
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Phenanthrenes / pharmacology*
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Phenanthrenes / therapeutic use
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Proteomics
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Transplantation, Heterologous
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Tripterygium / chemistry
Substances
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14-3-3 Proteins
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Antineoplastic Agents, Alkylating
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Diterpenes
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Epoxy Compounds
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Phenanthrenes
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triptolide