[Effect of extracts from Radix Trichosanthis on the expression of HBsAg and HBeAg in HepG2.2.15 cells]

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2012 Jan;37(1):38-41. doi: 10.3969/j.issn.1672-7347.2012.01.007.
[Article in Chinese]

Abstract

Objective: To investigate the effect of extracts with water and alcohol from Radix Trichosanthis on the cell survival and the expression of hepatitis B surface antigen (HBsAg) and hepatitis B e-antigen (HBeAg) in HepG2.2.15 cell supernatant.

Methods: The cell survival rate of HepG2.2.15 cells was detected by MTT assay. The HBsAg and HBeAg in HepG 2.2.15 cell supernatant were evaluated by enzyme linked immunosorbent assay.

Results: The water and alcohol soluble extracts from Radix Trichosanthis significantly inhibited the levels of HBsAg and HBeAg in HepG2.2.15 cells in a time-and-concentration-dependent manner. However, the therapeutic index of extracts with water from Radix Trichosanthis was better than that in the alcohol group.

Conclusion: The activity of water-soluble extract from Radix Trichosanthis is stronger on anti-hepatitis B virus than that of the alcohol-soluble extract.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antiviral Agents / pharmacology*
  • Drugs, Chinese Herbal / classification
  • Drugs, Chinese Herbal / pharmacology*
  • Hep G2 Cells
  • Hepatitis B Surface Antigens / biosynthesis*
  • Hepatitis B e Antigens / biosynthesis*
  • Hepatitis B virus / drug effects
  • Humans

Substances

  • Antiviral Agents
  • Drugs, Chinese Herbal
  • Hepatitis B Surface Antigens
  • Hepatitis B e Antigens