Gamma enolase expression as early marker of neuronal differentiation of murine neuroblastoma cells N-115

M Cervello; A Giallongo; L D'Amelio; S Sciarrino; V Matranga

doi:10.1007/BF00746095

Gamma enolase expression as early marker of neuronal differentiation of murine neuroblastoma cells N-115

Cytotechnology. 1993 Jan;11(Suppl 1):S167-9. doi: 10.1007/BF00746095.

Authors

M Cervello¹, A Giallongo, L D'Amelio, S Sciarrino, V Matranga

Affiliation

¹ Istituto di Biologia dello Sviluppo del CNR, via Archirafi 20, 90123, Palermo, Italy.

PMID: 22358696
DOI: 10.1007/BF00746095

Abstract

In this study we determined the levels of gamma enolase mRNA in mouse neuroblastoma cell line N-115 at early period of induction of differentiation by serum withdrawal. The expression of gamma enolase was examined by Northern blot analysis of total RNA extracted from cells induced for different lengths of time. We found a 3-fold increase in the level of gamma enolase mRNA after 24 hours of induction of differentiation and higher levels were detected in cells induced for longer time, reaching a 10-fold increase after four days.