Synthetic polymer nanoparticles conjugated with FimH(A) from E. coli pili to emulate the bacterial mode of epithelial internalization

J Am Chem Soc. 2012 Mar 7;134(9):3938-41. doi: 10.1021/ja2091917. Epub 2012 Feb 23.

Abstract

Amphiphilic block copolymer nanoparticles are conjugated with uropathogenic Escherichia coli type 1 pilus adhesin FimH(A) through amidation chemistry to enable bladder epithelial cell binding and internalization of the nanoparticles in vitro.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adhesins, Escherichia coli / chemistry*
  • Epithelial Cells / cytology*
  • Escherichia coli / chemistry*
  • Escherichia coli / cytology*
  • Fimbriae Proteins / chemistry*
  • Models, Molecular
  • Nanoparticles / chemistry*
  • Particle Size
  • Polymers / chemical synthesis*
  • Polymers / chemistry
  • Surface Properties

Substances

  • Adhesins, Escherichia coli
  • Polymers
  • fimH protein, E coli
  • Fimbriae Proteins