Partial small bowel resection with sleeve gastrectomy increases adiponectin levels and improves glucose homeostasis in obese rodents with type 2 diabetes

World J Surg. 2012 Jun;36(6):1432-8. doi: 10.1007/s00268-012-1483-6.

Abstract

Background: The aim of this study was to examine the effect of small bowel resection with and without sleeve gastrectomy on glucose homeostasis in an obese rodent model of type 2 diabetes.

Methods: Zucker diabetic fatty rats were randomized into three surgical groups: Sham, small bowel resection, and small bowel resection with sleeve gastrectomy (BRSG). Weight and fasting glucose levels were measured at randomization and monitored after surgery. Oral glucose tolerance testing was performed at baseline and 45 days after surgery to assess glucose homeostasis and peptide changes.

Results: At baseline, all animals exhibited impaired glucose tolerance and showed no difference in weight or fasting (area under the curve) AUC(glucose). At sacrifice, Sham animals weighed more than BRSG animals (p = 0.047). At day 45, the Sham group experienced a significant increase in AUC(glucose) compared to baseline (p = 0.02), whereas there was no difference in AUC(glucose) in either surgical group at any time point: BR (p = 0.58) and BRSG (p = 0.56). Single-factor ANOVA showed a significant difference in AUC(glucose) of p = 0.004 between groups postoperatively: Sham (50,745 ± 11,170) versus BR (23,865 ± 432.6) (p = 0.01); Sham versus BRSG (28,710 ± 3188.8) (p = 0.02). There was no difference in plasma insulin, GLP-1, or adiponectin levels before surgery, although 45 days following surgery adiponectin levels where higher in the BRSG group (p = 0.004).

Conclusions: Partial small bowel resection improved glucose tolerance independent of weight. The combination of small bowel resection and sleeve gastrectomy leads to an increase in adiponectin levels, which may contribute to improved glucose homeostasis.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Adiponectin / blood*
  • Animals
  • Biomarkers / blood
  • Blood Glucose / metabolism*
  • Diabetes Mellitus, Type 2 / blood
  • Diabetes Mellitus, Type 2 / complications
  • Diabetes Mellitus, Type 2 / surgery*
  • Gastrectomy / methods*
  • Glucagon-Like Peptide 1 / blood
  • Glucose Tolerance Test
  • Homeostasis*
  • Insulin / blood
  • Intestine, Small / surgery*
  • Male
  • Obesity / blood
  • Obesity / complications
  • Obesity / surgery*
  • Random Allocation
  • Rats
  • Rats, Zucker
  • Treatment Outcome
  • Weight Loss

Substances

  • Adiponectin
  • Biomarkers
  • Blood Glucose
  • Insulin
  • Glucagon-Like Peptide 1