Amyloid β-peptide neurotoxicity assay using cultured rat cortical neurons

Laura Facci; Stephen D Skaper

doi:10.1007/978-1-61779-536-7_6

Amyloid β-peptide neurotoxicity assay using cultured rat cortical neurons

Methods Mol Biol. 2012:846:57-65. doi: 10.1007/978-1-61779-536-7_6.

Authors

Laura Facci¹, Stephen D Skaper

Affiliation

¹ Department of Pharmacology and Anesthesiology, University of Padova, Padova, Italy.

PMID: 22367801
DOI: 10.1007/978-1-61779-536-7_6

Abstract

Neurons cultured from rodent central nervous system tissues represent an important tool in the study of neurodegenerative disease mechanisms and neuroregenerative processes, including the survival- and axon growth-promoting properties of neurotrophic factors. This chapter presents a detailed protocol for the preparation of rat and mouse cortical and hippocampal neuron cell cultures, using either embryonic or postnatal tissue.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphate / metabolism
Amyloid beta-Peptides / toxicity*
Animals
Cerebral Cortex / cytology*
Cerebral Cortex / embryology*
Enzyme Assays / methods
L-Lactate Dehydrogenase / metabolism
Luminescent Measurements / methods
Neurons / drug effects*
Neurons / metabolism
Rats

Substances

Amyloid beta-Peptides
Adenosine Triphosphate
L-Lactate Dehydrogenase