Central nervous system neuron-glia co-culture models

Methods Mol Biol. 2012:846:79-89. doi: 10.1007/978-1-61779-536-7_8.

Abstract

Glial cell activation plays an important role in the pathogenesis of various neurodegenerative disorders. This article presents a protocol for the preparation of cultures consisting of rat embryonic cortical neurons grown in the presence of cortical microglia, in which the glia are present in physical contact with the neurons or separated by a semi-permeable membrane barrier.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques / methods*
  • Cell Separation / methods*
  • Central Nervous System / cytology*
  • Embryo, Mammalian / cytology
  • L-Lactate Dehydrogenase / metabolism
  • Neuroglia / cytology*
  • Neurons / cytology*
  • Rats

Substances

  • L-Lactate Dehydrogenase