Administration of poly[di(sodium carboxylatoethylphenoxy)]phosphazene (PCEP) as adjuvant activated mixed Th1/Th2 immune responses in pigs

Arshud Dar; Ken Lai; Donna Dent; Andrew Potter; Volker Gerdts; Lorne A Babiuk; George K Mutwiri

doi:10.1016/j.vetimm.2012.01.021

Administration of poly[di(sodium carboxylatoethylphenoxy)]phosphazene (PCEP) as adjuvant activated mixed Th1/Th2 immune responses in pigs

Vet Immunol Immunopathol. 2012 May 15;146(3-4):289-95. doi: 10.1016/j.vetimm.2012.01.021. Epub 2012 Feb 6.

Authors

Arshud Dar¹, Ken Lai, Donna Dent, Andrew Potter, Volker Gerdts, Lorne A Babiuk, George K Mutwiri

Affiliation

¹ Vaccine and Infectious Disease Organization, Department of Veterinary Microbiology, University of Saskatchewan, 120-Veterinary Road, Saskatoon, SK, Canada S7N 5E3. [email protected]

PMID: 22377627
DOI: 10.1016/j.vetimm.2012.01.021

Abstract

The selection of an optimal adjuvant to enhance the potency and longevity of antigen specific immune responses has always been imperative for the development of more effective and safer vaccines. A balanced type of immune enhancing ability of a new adjuvant known as polyphosphazene (PCEP) has been demonstrated in mice. In the present study we have compared the humoral and cellular immune responses to vaccine formulations containing Actinobacillus pleuropneumoniae outer membrane antigen (OmlA) with PCEP or Emulsigen as adjuvants. Our data showed a significant improvement and a shift toward more balanced immune responses when OmlA antigen was formulated with PCEP and CpG ODN. Moreover, in contrast to Emulsigen, immunization with PCEP did not result in local injection site reactions highlighting its potential as a safe and effective adjuvant for pigs. Further, the ease of formulation, administration and relatively low per dose cost of PCEP make it a promising adjuvant for pigs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actinobacillus Infections / immunology
Actinobacillus Infections / microbiology
Actinobacillus Infections / prevention & control
Actinobacillus Infections / veterinary*
Actinobacillus pleuropneumoniae / immunology*
Adjuvants, Immunologic / pharmacology*
Animals
Antibodies, Bacterial / blood
Bacterial Outer Membrane Proteins / genetics
Bacterial Outer Membrane Proteins / immunology*
Bacterial Vaccines / administration & dosage
Bacterial Vaccines / genetics
Bacterial Vaccines / immunology*
Enzyme-Linked Immunosorbent Assay / veterinary
Lipoproteins / genetics
Lipoproteins / immunology*
Phenylpropionates / pharmacology*
Polymers / pharmacology*
Swine
Swine Diseases / immunology*
Swine Diseases / microbiology
Swine Diseases / prevention & control
Th1 Cells / immunology
Th2 Cells / immunology
Vaccination / methods
Vaccination / veterinary

Substances

Adjuvants, Immunologic
Antibodies, Bacterial
Bacterial Outer Membrane Proteins
Bacterial Vaccines
Lipoproteins
OmlA protein, bacteria
Phenylpropionates
Polymers
poly(bis(carboxylatoethylphenoxy)phosphazene)