The composition of the culture medium influences the β-1,3-glucan metabolism of Aspergillus fumigatus and the antifungal activity of inhibitors of β-1,3-glucan synthesis

Antimicrob Agents Chemother. 2012 Jun;56(6):3428-31. doi: 10.1128/AAC.05661-11. Epub 2012 Mar 5.

Abstract

In vitro testing of Aspergillus fumigatus susceptibility to echinocandins has always been a challenge. Using a simple and quick colorimetric method to analyze the activity of inhibitors of β-1,3-glucan synthesis, we found that the composition of the culture medium significantly influences glucan synthesis and consequently the antifungal properties of inhibitors of β-1,3-glucan synthesis when they are tested alone or in combination with chitin synthase inhibitors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antifungal Agents / pharmacology*
  • Aspergillus fumigatus / drug effects*
  • Aspergillus fumigatus / metabolism*
  • Caspofungin
  • Culture Media / pharmacology*
  • Echinocandins / pharmacology
  • Glycosides / pharmacology
  • Lipopeptides
  • Miconazole / pharmacology
  • Microbial Sensitivity Tests
  • Triterpenes / pharmacology
  • beta-Glucans / metabolism*

Substances

  • Antifungal Agents
  • Culture Media
  • Echinocandins
  • Glycosides
  • Lipopeptides
  • Triterpenes
  • beta-Glucans
  • enfumafungin
  • Miconazole
  • beta-1,3-glucan
  • Caspofungin