Extrahepatic bile duct regeneration in pigs using collagen scaffolds loaded with human collagen-binding bFGF

Qiang Li; Liang Tao; Bing Chen; Haozhen Ren; Xianglin Hou; Siqiao Zhou; Jianxin Zhou; Xitai Sun; Jianwu Dai; Yitao Ding

doi:10.1016/j.biomaterials.2012.03.003

Extrahepatic bile duct regeneration in pigs using collagen scaffolds loaded with human collagen-binding bFGF

Biomaterials. 2012 Jun;33(17):4298-308. doi: 10.1016/j.biomaterials.2012.03.003. Epub 2012 Mar 18.

Authors

Qiang Li¹, Liang Tao, Bing Chen, Haozhen Ren, Xianglin Hou, Siqiao Zhou, Jianxin Zhou, Xitai Sun, Jianwu Dai, Yitao Ding

Affiliation

¹ Department of Hepatobiliary Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China.

PMID: 22429981
DOI: 10.1016/j.biomaterials.2012.03.003

Abstract

Extrahepatic bile duct defects and their complications are benign lesions but with malignant outcomes. Extrahepatic bile duct regeneration at the injury site could be important for the repair. In our previous work, a human basic fibroblast growth factor (bFGF) fused with a collagen-binding domain (CBD) was produced to activate the collagen membrane to obtain targeted tissue regeneration. This collagen/growth factor functional biomaterial could promote the regeneration of skin, bladder and full-thickness abdominal wall by accelerating vascularization and cellularization of autologous tissues. We speculate that the functional biomaterial could also provide the repairing effect on extrahepatic bile duct injuries. Using a pig extrahepatic bile duct injury model, we found that the collagen/CBD-bFGF composite biomaterial could significantly promote the extrahepatic bile duct regeneration at the injury site without causing structure deformation or hepatic dysfunction during both short- and long-time observations.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bile Ducts, Extrahepatic / diagnostic imaging
Bile Ducts, Extrahepatic / pathology
Bile Ducts, Extrahepatic / physiology*
Bile Ducts, Extrahepatic / surgery
Bilirubin / metabolism
Body Weight / drug effects
Cattle
Cholangiography
Collagen / metabolism
Collagen / pharmacology*
Collagen / ultrastructure
Constriction, Pathologic
Desmin / metabolism
Fibroblast Growth Factor 2 / metabolism
Fibroblast Growth Factor 2 / pharmacology*
Humans
Implants, Experimental
Keratin-19 / metabolism
Liver / drug effects
Liver / enzymology
Liver Function Tests
Myocytes, Smooth Muscle / drug effects
Myocytes, Smooth Muscle / metabolism
Neovascularization, Physiologic / drug effects
Plastic Surgery Procedures
Postoperative Complications / etiology
Postoperative Complications / pathology
Protein Binding / drug effects
Regeneration / drug effects*
Staining and Labeling
Sus scrofa
Time Factors
Tissue Scaffolds / chemistry*
von Willebrand Factor / metabolism

Substances

Desmin
Keratin-19
von Willebrand Factor
Fibroblast Growth Factor 2
Collagen
Bilirubin