Carbon nanotubes induce bone calcification by bidirectional interaction with osteoblasts

Adv Mater. 2012 Apr 24;24(16):2176-85. doi: 10.1002/adma.201103832. Epub 2012 Mar 22.

Abstract

Multi-walled carbon nanotubes (MWCNTs) promote calcification during hydroxyapatite (HA) formation by osteoblasts. Primary cultured osteoblasts are incubated with MWCNTs or carbon black. After culture for 3 weeks, the degree of calcification is very high in the 50 μg mL(-1) MWCNT group. Transmission electron microscopy shows needle-like crystals around the MWCNTs, and diffraction patterns reveal that the peak of the crystals almost coincides with the known peak of HA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone and Bones / cytology*
  • Bone and Bones / drug effects
  • Bone and Bones / physiology*
  • Calcification, Physiologic / drug effects*
  • Cell Differentiation / drug effects
  • Cell Proliferation / drug effects
  • Humans
  • Male
  • Mice
  • NIH 3T3 Cells
  • Nanotubes, Carbon*
  • Osteoblasts / cytology
  • Osteoblasts / drug effects*

Substances

  • Nanotubes, Carbon