Ugi reaction-assisted rapid assembly of affinity-based probes against potential protein tyrosine phosphatases

Jingyan Ge; Xiamin Cheng; Lay Pheng Tan; Shao Q Yao

doi:10.1039/c2cc31294h

Ugi reaction-assisted rapid assembly of affinity-based probes against potential protein tyrosine phosphatases

Chem Commun (Camb). 2012 May 11;48(37):4453-5. doi: 10.1039/c2cc31294h. Epub 2012 Mar 27.

Authors

Jingyan Ge¹, Xiamin Cheng, Lay Pheng Tan, Shao Q Yao

Affiliation

¹ Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.

PMID: 22451009
DOI: 10.1039/c2cc31294h

Abstract

The multi-component Ugi reaction has been employed to assemble a small library of affinity-based probes (AfBPs) that target potential protein tyrosine phosphatases. The probes showed good labelling of PTP1B and MptpB, and were subsequently used to label endogenous PTP1B in MCF-7 cell lysates.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Affinity Labels / chemical synthesis*
Affinity Labels / chemistry
Affinity Labels / metabolism*
Cell Line, Tumor
Drug Design*
Humans
Kinetics
Protein Tyrosine Phosphatases / metabolism*

Substances

Affinity Labels
Protein Tyrosine Phosphatases