Abstract
Dendritic cells (DC) are antigen-presenting cells found in both lymphoid and nonlymphoid organs, including the brain (bDC) of Cd11c/eyfp transgenic C57BL/6 mice. Using an intranasal vesicular stomatitis virus infection, we demonstrated that EYFP(+) cells amass in areas associated with viral antigens, take on an activated morphology, and project their processes into infected neuronal tissue within the olfactory bulb. These bDC separated into three EYFP(+) CD45(+) CD11b(+) populations, all but one being able to functionally promote both T lymphocyte proliferation and T(H)1 cytokine production. One population was shown to emanate from the brain and a second population was peripherally derived. The third population was of indeterminate origin, being both radiosensitive and not replenished by donor bone marrow. Finally, each EYFP(+) population contained CD11b(+) CD103(+) subpopulations and could be distinguished in terms of CD115, Gr-1, and Ly-6C expression, highlighting mucosal and monocyte-derived DC lineages.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigen Presentation / immunology
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Antigens, CD / immunology
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Antigens, CD / metabolism
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Antigens, Ly / immunology
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Antigens, Ly / metabolism
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Brain / immunology*
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Brain / metabolism
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Brain / virology
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CD11b Antigen / immunology
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CD11b Antigen / metabolism
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Cells, Cultured
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Dendritic Cells / immunology*
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Dendritic Cells / metabolism
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Encephalitis, Viral / genetics
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Encephalitis, Viral / immunology*
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Encephalitis, Viral / metabolism
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Flow Cytometry
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Integrin alpha Chains / immunology
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Integrin alpha Chains / metabolism
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Leukocyte Common Antigens / immunology
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Leukocyte Common Antigens / metabolism
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Microscopy, Confocal
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Olfactory Bulb / immunology*
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Olfactory Bulb / metabolism
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Ovalbumin / immunology
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Receptor, Macrophage Colony-Stimulating Factor / immunology
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Receptor, Macrophage Colony-Stimulating Factor / metabolism
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Receptors, Chemokine / immunology
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Receptors, Chemokine / metabolism
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Rhabdoviridae Infections / genetics
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Rhabdoviridae Infections / immunology
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Rhabdoviridae Infections / metabolism
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T-Lymphocytes / immunology
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T-Lymphocytes / metabolism
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Vesicular stomatitis Indiana virus / immunology
Substances
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Antigens, CD
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Antigens, Ly
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CD11b Antigen
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Gr-1 protein, mouse
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Integrin alpha Chains
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Luminescent Proteins
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Ly-6C antigen, mouse
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Receptors, Chemokine
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alpha E integrins
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Ovalbumin
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Receptor, Macrophage Colony-Stimulating Factor
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Leukocyte Common Antigens
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Ptprc protein, mouse