Hydrogen exchange mass spectrometry: are we out of the quicksand?

J Am Soc Mass Spectrom. 2012 Jun;23(6):1003-10. doi: 10.1007/s13361-012-0377-z. Epub 2012 Apr 3.

Abstract

Although the use of hydrogen exchange (HX) mass spectrometry (MS) to study proteins and protein conformation is now over 20 years old, the perception lingers that it still has "issues." Is this method, in fact, still in the quicksand with many remaining obstacles to overcome? We do not think so. This critical insight addresses the "issues" and explores several broad questions including, have the limitations of HX MS been surmounted and has HX MS achieved "indispensable" status in the pantheon of protein structural analysis tools.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Deuterium Exchange Measurement / methods*
  • Mass Spectrometry / methods*
  • Protein Conformation
  • Proteins / analysis
  • Proteins / chemistry*
  • Reproducibility of Results

Substances

  • Proteins