Linking differences in membrane tension with the requirement for a contractile actomyosin scaffold during exocytosis in salivary glands

In all the major secretory organs regulated exocytosis is a fundamental process that is used for releasing molecules in the extracellular space. Molecules destined for secretion are packaged into secretory vesicles that fuse with the plasma membrane upon the appropriate stimulus. In exocrine glands, large secretory vesicles fuse with specialized domains of the plasma membrane, which form ductal structures that are in direct continuity with the external environment and exhibit various architectures and diameters. In a recent study, we used intravital microscopy to analyze in detail the dynamics of exocytic events in the salivary glands of live rodents under conditions that cannot be reproduced in in vitro or ex vivo model systems. We found that after the opening of the fusion pore large secretory vesicles gradually collapse with their limiting membranes being completely absorbed into the apical plasma membrane canaliculi within 40-60 sec. Moreover, we observed that this controlled collapse requires the contractile activity of actin and its motor myosin II, which are recruited onto the large secretory vesicles immediately after their fusion with the plasma membrane. Here we suggest that the actomyosin complex may be required to facilitate exocytosis in those systems, such as the salivary glands, in which the full collapse of the vesicles is not energetically favorable due to a difference in membrane tension between the large secretory vesicles and the canaliculi.