Abstract
The development of thermo-responsive and reduction-sensitive polymeric micelles based on an amphiphilic block copolymer poly[(PEG-MEMA)-co-(Boc-Cyst-MMAm)]-block-PEG (denoted PEG-P-SS-HP) for the intracellular delivery of anticancer drugs is reported. PTX, as model drug, was loaded into the PEG-P-SS-HP micelles with an encapsulation efficiency >90%, resulting in a high drug loading content (up to 35 wt%). The PTX-loaded PEG-P-SS-HP micelles show slow drug release in PBS and rapid release after incubation with DTT. The PTX-loaded micelles display a better cytotoxic effect than the free drug, whereas empty micelles are found to be non-toxic. The thermo-responsive and reduction-sensitive polymeric micelles described may serve as promising carriers for cytostatic drugs.
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acrylic Resins / chemical synthesis*
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Acrylic Resins / pharmacology
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Antineoplastic Agents, Phytogenic / chemistry
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Antineoplastic Agents, Phytogenic / pharmacology*
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Biocompatible Materials / chemical synthesis*
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Biocompatible Materials / pharmacology
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Cell Survival / drug effects
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Dithiothreitol / chemistry
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Drug Carriers / chemical synthesis*
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Drug Carriers / pharmacology
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Drug Compounding
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Hep G2 Cells
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Humans
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Magnetic Resonance Spectroscopy
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Micelles
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Oxidation-Reduction
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Paclitaxel / chemistry
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Paclitaxel / pharmacology*
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Polyethylene Glycols / chemical synthesis*
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Polyethylene Glycols / pharmacology
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Polymers / chemical synthesis*
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Polymers / pharmacology
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Temperature
Substances
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Acrylic Resins
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Antineoplastic Agents, Phytogenic
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Biocompatible Materials
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Drug Carriers
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Micelles
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Polymers
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poly((poly(ethylene glycol) methyl methacrylate)-co-(tert-butyloxycarbonylaminoethyldithioethyl methacrylamide))
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Polyethylene Glycols
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Paclitaxel
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Dithiothreitol