Using soybean trypsin inhibitor as an external loading control for Western blot analysis of tear proteins: application to corneal disease

Exp Eye Res. 2012 Jun:99:55-62. doi: 10.1016/j.exer.2012.03.012. Epub 2012 Apr 4.

Authors

Jingjing You¹, Chris Hodge, Li Wen, John W McAvoy, Michele C Madigan, Gerard Sutton

Affiliation

¹ Save Sight Institute, Discipline of Ophthalmology, University of Sydney, GPO Box 4337, Sydney NSW 2001, Australia. [email protected]

PMID: 22498032
DOI: 10.1016/j.exer.2012.03.012

No abstract available

MeSH terms

Actins / metabolism
Adult
Blotting, Western*
Electrophoresis, Polyacrylamide Gel
Eye Proteins / metabolism*
Female
Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / metabolism
Glycine max
Humans
Intercellular Signaling Peptides and Proteins / metabolism*
Keratoconus / metabolism*
Male
Membrane Proteins / metabolism*
Quality Control*
Soybean Proteins / chemistry*
Tears / metabolism
Trypsin Inhibitors / chemistry*
Tubulin / metabolism

Substances

Actins
Eye Proteins
Intercellular Signaling Peptides and Proteins
Membrane Proteins
SFRP1 protein, human
Soybean Proteins
Trypsin Inhibitors
Tubulin
tear proteins
Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)