[Study on tissue culture and plant regeneration of the stem-tips and buds of Periploca forrestii]

Zhong Yao Cai. 2011 Nov;34(11):1656-60.
[Article in Chinese]

Abstract

Objective: To establish the rapid propagation systems of the stem-tips and buds of Periploca forrestii.

Methods: Inserted the stem-tips and buds of Periploca forrestii into MS medium with different concentrations of 6-BA, NAA and 2.4-D and induced them growing into complete plants.

Results and conclusion: The optimal culture medium for bud induction is MS + 6-BA 1.0 mg/L + NAA 0.3 mg/L and the bud induction rate can reach 86.29%. The optimal culture medium for stem-tips induction is MS +6-BA 2.0 mg/L + NAA 0.5 mg/L and the bud induction rate can reach 86.29%. The optimal culture medium for bud multiplication is MS + 6-BA 2.0 mg/L + NAA 0.1 mg/L and the multiplication coefficient can reach 2.10. The best rooting medium is 1/2 MS + IBA 0.5 mg/L and the rooting rate is 53.33%.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Culture Media
  • Periploca / growth & development*
  • Plant Growth Regulators / pharmacology
  • Plant Shoots / growth & development
  • Plant Stems / growth & development
  • Plants, Medicinal / growth & development*
  • Regeneration*
  • Tissue Culture Techniques / methods*

Substances

  • Culture Media
  • Plant Growth Regulators