The treatment of human immunodeficiency virus (HIV)-infected pregnant women is one of the most effective HIV-prevention interventions known. With prenatal testing, antiviral therapy, scheduled cesarean section when indicated, and formula feeding when feasible, rates of neonatal and perinatal HIV infection can diminish significantly. Although significant prevention is achievable, new cases of HIV in neonates continue to occur both worldwide and locally due to limited resources, delayed diagnosis, lack of linkage to care, and lack of timely effective therapy. Where those resources are available, the management of HIV infection in the pregnant woman requires a multidisciplinary expert approach to achieve optimal outcomes for both mother and child.