HTLV-I virological and histopathological analysis in two cases of anti-centromere-antibody-seropositive Sjögren's syndrome

Mod Rheumatol. 2013 Jan;23(1):133-9. doi: 10.1007/s10165-012-0641-x. Epub 2012 Apr 18.

Abstract

Introduction: The aim of this study was to show the clinical and pathological characteristics of anti-centromere-antibody (ACA)-seropositive Sjögren's syndrome (SS) in two anti-human T-cell leukemia virus type I (HTLV-I)-seropositive patients.

Methods: One patient was an HTLV-I carrier whereas the other was diagnosed with HTLV-I-associated myelopathy (HAM). Background data including serum HTLV-I titers, viral loads, and cytokine profiles were recorded. Azocarmine with aniline blue (Azan)-Mallory staining and immunohistochemistry of the labial salivary glands (LSGs) and a muscle biopsy specimen from the HAM patient were performed.

Results: Serum transforming growth factor beta (TGF-β), tumor necrosis factor alpha (TNF-α), and HTLV-I viral load were high in the HAM-SS patient compared with the HTLV-I carrier. Fibrous change in LSG was prominent in the HAM-SS patient. Although TGF-β expression was similar in the two patients, expression of HTLV-I-related proteins including p12, p28, group-specific antigen (GAG), and nuclear factor kappa-B (NF-κB) in the LSG were dominantly detected in the HAM-SS patient. Frequency of TGF-β staining in HTLV-I-seropositive SS patients without ACA, HTLV-I-seronegative SS patients with ACA, and HTLV-I-seronegative SS patients without ACA was lower than that of the previous two patients.

Conclusion: A high HTLV-I viral load in situ is supposed to promote the production of cytokines, especially TGF-β, resulting in the fibrous change of LSG in ACA-seropositive SS patients.

Publication types

  • Case Reports

MeSH terms

  • Antibodies, Antinuclear / blood*
  • Biomarkers / metabolism
  • Carrier State
  • Centromere / immunology*
  • Female
  • Fibrosis / pathology
  • HTLV-I Infections / blood
  • HTLV-I Infections / complications*
  • HTLV-I Infections / pathology*
  • Human T-lymphotropic virus 1 / isolation & purification
  • Human T-lymphotropic virus 1 / physiology
  • Humans
  • Lip
  • Middle Aged
  • Mouth Mucosa
  • Muscle, Skeletal / metabolism
  • Muscle, Skeletal / pathology
  • Salivary Glands, Minor / metabolism
  • Salivary Glands, Minor / pathology
  • Salivary Glands, Minor / virology
  • Sjogren's Syndrome / blood
  • Sjogren's Syndrome / pathology*
  • Sjogren's Syndrome / virology*
  • Transforming Growth Factor alpha / blood
  • Transforming Growth Factor beta / blood
  • Viral Load
  • Viral Proteins / metabolism
  • Xerostomia / diagnosis
  • Xerostomia / etiology

Substances

  • Antibodies, Antinuclear
  • Biomarkers
  • Transforming Growth Factor alpha
  • Transforming Growth Factor beta
  • Viral Proteins