Cumulus cell-oocyte complexes retrieved from antral follicles in IVM cycles: relationship between COCs morphology, gonadotropin priming and clinical outcome

J Assist Reprod Genet. 2012 Jun;29(6):513-9. doi: 10.1007/s10815-012-9766-2. Epub 2012 Apr 18.

Abstract

Purpose: To assess retrospectively the developmental potential of different types of cumulus cell-oocyte complexes (COCs) derived from IVM cycles.

Methods: IVM cycles were performed in natural cycles or after HCG, FSH, or FSH/HCG priming. COCs recovered were morphologically characterized in different types: compact (CC) or expanded (EC) cumulus mass but including an immature oocyte, and expanded cumulus mass enclosing a mature oocyte (EC-MII). Embryo developmental competence was investigated analysing exclusively cycles in which all transferred embryos derived from the same COC category.

Results: Fertilization rates did not differ significantly. Significant differences in pregnancy rates (14.5%, 10.0% and 27.6 % in the CC, EC, and EC-MII categories, respectively) were observed. Likewise, significant differences in implantation rates (8.9%, 6.3% and 19.1% in the CC, EC, and EC-MII categories, respectively) were found. Overall, priming with FSH/HCG had a beneficial effect on pregnancy and implantation rates, while no priming or HCG alone generated oocytes with poor competence.

Conclusions: In IVM cycles, morphological evaluation at the time of collection can predict the developmental ability of different COCs. FSH/HGC priming has a positive effect on oocyte competence.

Publication types

  • Clinical Trial

MeSH terms

  • Chorionic Gonadotropin / pharmacology*
  • Chorionic Gonadotropin / therapeutic use
  • Cumulus Cells / cytology*
  • Cumulus Cells / physiology
  • Embryo Transfer
  • Embryonic Development
  • Female
  • Fertilization in Vitro*
  • Follicle Stimulating Hormone / pharmacology*
  • Follicle Stimulating Hormone / therapeutic use
  • Humans
  • Oocytes / cytology*
  • Oocytes / drug effects
  • Oocytes / physiology
  • Ovarian Follicle / cytology*
  • Ovarian Follicle / drug effects*
  • Pregnancy
  • Pregnancy Rate
  • Retrospective Studies
  • Treatment Outcome

Substances

  • Chorionic Gonadotropin
  • Follicle Stimulating Hormone