Isolation and characterization of microsatellite markers for Plathymenia reticulata (Fabaceae)

Am J Bot. 2012 May;99(5):e210-2. doi: 10.3732/ajb.1100511. Epub 2012 Apr 26.

Abstract

Premise of the study: Microsatellite loci were isolated and characterized for use in population genetic studies of Plathymenia reticulata (Fabaceae), a tropical tree widespread in the Atlantic Forest and cerrado biomes of South America.

Methods and results: Nine microsatellite markers were developed using a simple sequence repeat-enriched library. Polymorphism was analyzed in 51 individuals from two populations. All loci were polymorphic, with the number of alleles per loci ranging from five to 15 (mean number of alleles: 10.22). Observed and expected heterozygosities per loci and population ranged from 0.313 to 1.000 and 0.280 to 0.869, respectively.

Conclusions: These highly informative loci are potentially useful to estimate population genetic structure and to understand evolutionary processes and taxonomy of the species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brazil
  • DNA Primers / metabolism
  • DNA, Plant / genetics*
  • DNA, Plant / isolation & purification*
  • Fabaceae / genetics*
  • Genetic Loci / genetics
  • Microsatellite Repeats / genetics*
  • Molecular Sequence Data

Substances

  • DNA Primers
  • DNA, Plant

Associated data

  • GENBANK/JN840010
  • GENBANK/JN840011
  • GENBANK/JN840012
  • GENBANK/JN840013
  • GENBANK/JN840014
  • GENBANK/JN840015
  • GENBANK/JN840016
  • GENBANK/JN840017
  • GENBANK/JN840018